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Starting with skin fibroblasts from patients diagnosed with cystic fibrosis, we derived and characterized induced pluripotent stem cell (iPSC) lines.
As the EMS mutants are nontransgenic, subsequent generations can be grown under field conditions, without restrictions, for phenotypic analysis and advantageous alleles can be immediately incorporated into a fast-paced molecular breeding program using the characterized induced mutations as markers for selection.
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We have used HrpNEa, the first-characterized [ 1] and well-studied harpin [ 2- 7], as a model of proteinaceous elicitors to characterize induced resistance against insect herbivores [ 2, 4, 8, 34], particularly the green peach aphid M. persicae, a generalist phloem-feeding insect [ 10].
Here, DNA-AFLP and MSAP fingerprinting were applied to characterize induced changes in the genome and epigenome, and cDNA-AFLP were used to detect changes to the transcriptome in newly synthesized C. nankingense × Tanacetum vulgare and C. crassum × Crossostephium chinense hybrids.
Here, we have characterized light induced gene transcription in the zebrafish at several organizational levels.
We have recently reported the presence of an ovulation-inducing factor (OIF) in the seminal plasma of llamas and alpacas species characterized as induced ovulators.
USC were isolated, characterized and induced to differentiate into UC and SMC.
Printing time effect has been also characterized and induced a change in the capillary bridge shape.
"Typically it takes more than one to two months--to start from human skin fibroblasts or blood cells, to a well characterized, established induced pluripotent stem cell culture -- at least a couple of months," said Dr. Sheng Ding.
In fact Gto2, recently characterized, was induced by oxidative stress [13].
Although ER stress was initially characterized as induced by accumulation of unfolded proteins [11] [13], it remains impossible to quantitate the levels of stress inflicted by accumulation of misfolded proteins in the ER.
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