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The films were first characterized immediately after their preparation and afterwards were allowed to oxidize in air at room temperature for a given time to study their aging.
The cell was infiltrated with a liquid I3 -/I- electrolyte containing 0.1 M LiI, 50 mM I2 and 0.6 M 1,2-dimethyl-3-propylimidazolium iodide dissolved in acetonitrile, sealed, and characterized immediately owing to the low stability of CdS and CdSe QDs in I3 -/I- electrolyte [20].
Cells were harvested every 12 hr, concentrated via centrifugation, and characterized immediately via flow cytometry.
The devices were characterized immediately after the illumination in an argon glove box with a Parametric Analyzer (Agilent Technologies (Santa Clara, USA) – E5262A).
Cell products were evaluated by standard sterility tests for gram+, gram-, andobic anaerobicobacteriaeria, mycetes and with endotoxins tests, and characterized immediately by Flow Cytometry (FC) for EpCAM (Miltenyi Biotec, CD326/EpCAM-FITC, human; dilution 1 50) and LGR5 (OriGene, LGR5-PE, human; dilution 1 50) before transplantation in the patients.
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After gently washing with buffer (10 min, 2 cycles), samples were immediately characterized by confocal microscopy and AFM.
All samples were collected manually in 20 l plastic containers, and then transferred, characterized, and refrigerated immediately in accordance with the Standard Methods for the Examination of Water and Wastewater (APHA 2005).
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