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They were characterized for gene expression by qRT-PCR.
A recent study reported the identification of 7,009 unigenes from a normalized cDNA library of jatropha developing seeds and, of which, 17 genes encoding enzymes for fatty acid and lipid biosynthesis were further characterized for gene expression by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) [ 8].
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These were crossed to UAS reporter lines and progeny were characterized for reporter gene expression as a function of temperature.
Our initial aim was to demonstrate the use of universal RNA standards to characterize different methods for gene expression quantification and provide technical information which can be applied to mRNA biomarkers of differing levels of abundance.
The NCI-60 panel has been extensively molecularly characterized, with data available for gene expression, DNA variation (mutation and SNPs), protein expression, DNA methylation, microRNA expression and metabolomics (http://dtp.cancer.gov/mtargets/mt_index.html) [ 3– 9].
With the rapid development of high-throughput genomic technologies and the accumulation of genome-wide datasets for gene expression profiling and biological networks, the impact of diseases and drugs on gene expression can be comprehensively characterized.
The availability of representative cell lines characterized for the gene-expression profiles is a unique opportunity to establish the cellular/molecular determinants of retinoid sensitivity in mammary tumors.
In the present study, by analyzing multiple female goldfish brain microarray datasets, we have characterized global gene expression patterns for a seasonal cycle.
To identify additional genes that might be involved in PDA initiation and tumor growth, and therefore be potential targets for drug treatment, we characterized the gene expression profile of primary PDA cells in culture by RNA-Seq.
We previously characterized the gene expression response to rapamycin for the WB-F344 and WB311 hepatic cell lines [18].
The ESTs generated from the present study, especially those from full-length enriched cDNA libraries, will be a useful resource for the ongoing melon whole genome sequencing project and for characterizing gene expression patterns and traits of interest in melon and closely related species.
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