Polymeric nanocapsules (NC) and nanospheres (NS), nanostructured lipid carriers (NLC) and liposomes were prepared and characterized for size, distribution, zeta potential and number per volume of the colloidal dispersion.
By an emulsion-evaporation method the nanospheres were produced and then characterized for size distribution, zeta-potential, in vitro drug release profile and cellular studies.
Insertion sites were characterized for their distribution throughout the genome and proximity to genes (Additional file 2: Dataset S1).
Freeze-dried NS were resuspended with filtered water and were characterized for size distribution, surface chemistry and technological parameters.
Each BAC library was characterized for insert distribution by randomly selecting 384 clones and subjecting them to miniprep, digestion with Not-I (New England Biolabs), and resolution by pulsed-field gel electrophoresis [ 23]. 384-well plates containing BACs were decondensed to 96-well format robotically with the Q-bot (Genetix, United Kingdom).
Batches were characterized for particle size distribution, density (porosity), flow, compaction, and dissolution rate.
The microspheres were also characterized for particle size/size distribution, thermal properties and morphology.
Particles were first characterized for size, size distribution, surface area, iron concentration, phase and aggregation in cell culture media.
Particles were characterized for morphology, size distribution, surface charge and cytocompatibility, as well as release of transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α (TNF-α).
Uncoated and coated particles were characterized for morphology, size distribution, and surface charge.
