Second, we describe a set of newly characterized EST-SSR loci and provide information about their polymorphism, Mendelian inheritance, and transferability to other Silene species.
Indeed, the P. falciparum genome is estimated to possess more than 300,000 TTAA sites with ~40% appearing in all characterized ESTs yielding an average of >20 integration sites per gene [ 13].
Though we briefly touched on these questions by using independent genotypes for SNP validation, these questions are out of the scope of this particular study that aims to primarily characterize EST sequences of big sagebrush and provide insight regarding the origins of ssp.
The objectives of this study were to (i) increase the number of SSR markers by mining a previously developed cassava 8,577 unigene set; (ii) characterize EST-derived microsatellite markers in terms of abundance and level of polymorphism; and (iii) assess their transferability to other taxa in the Euphorbiaceae.
Those motifs with more than 10 repeats were characterized in EST sequences of this family, which occupied 91.65% (6,588 markers) of total 7,188 SSR markers.
In previous studies, the expression profiles of wood formation have been characterized by EST (expressed sequence tags) sequencing and microarry hybridization in poplar [ 3- 6], Eucalyptus [ 7, 8], Pinus [ 9, 10] and spruce [ 11].
We constructed a cDNA library of D. magna and characterized the ESTs of over 7,000 clones (Watanabe et al. 2005).
Previous studies have characterized some EST-SSR in clams using 454 [ 26, 32] but none used R. decussatus as a model.
We sequenced and characterized 4394 ESTs (1524 from liver, 1451 from head kidney and 1419 from skeletal muscle) from three "forward subtracted" libraries (enriched for genes up-regulated by heat-shock) and 1586 from the liver "reverse subtracted" library (enriched for genes down-regulated by heat-shock), for a total of 5980 ESTs.
The 446 high quality ESTs from the ZEA library represented 334 unigene EST sets, BLAST searches returned 412 ESTs with similarity to characterized proteins, 4 ESTs matched hypothetical proteins and 30 ESTs showed no similarity.
