NMR relaxation studies of proteins to date have focused primarily on characterizing backbone dynamics.
Our results indicate that the R5 labeling scheme may prove to be a valuable tool for characterizing backbone dynamics in large RNAs.
DOI: http://dx.doi.org/10.7554/eLife.07888.014 > > -wrap-foot> To further characterize differences in structure and dynamics of PLK4-PB3 seen upon STIL-CC binding in aqueous solution, free and STIL-CC-bound PLK4-PB3 were subjected to 2D [N,H]-TROSY experiments to reveal chemical shift perturbations and to N-{H}NOE measurements to characterize backbone dynamics on the ps- to ns-timescale.
We first characterized the backbone flexibility of apo I28A, by measuring backbone amide N relaxation parameters, R1, R2, and steady-state N H NOE at 16.4 T, 295 K.
Based on these insights, analytical equations are proposed for characterizing the backbone curve of the hysteretic response, for use in displacement based design methods.
Sativene sesquiterpenoids, as well as its derivatives, usually possessed an unusual backbone characterized by a 5/6 or 5/7 bridge ring system [1, 2, 3, 4, 5, 6, 7], which have become targets of synthetic chemists [8, 9, 10, 11, 12].
These ligands have the following properties: chitosan is a linear polysaccharide with a backbone characterized by a random distribution of β- 1-4 -linked d-glucosamine and N-acetyl-d-glucosamine units.
It was characterized that the backbone rigidity between two catechol substructures was required for the full activity and acid substructure of the lead compound was important for the activity.
Also, there are no available 3D structures of Rhizobium nodulation factors, which are characterized by a backbone of between three and five substituted (sulfonated) GlcNAc residues.
