From the poor characterized annotations, General function prediction only represented 18.02% and Function unknown represented 5.62%, which is quite expected since C. pictus is remotely similar to the organisms originally present in the eukaryotic KOG database.
The DEGs were characterized by annotation using the Gene Ontology (GO) database [ 31].
To further characterized sequence annotation, all unigenes were searched against COG database to analyze phylogenetically widespread domain families.
Three databases UniProt (Apweiler et al. 2004), CHEBI (Degtyarenko et al. 2008), and PubChem (Wang et al. 2009) were used to characterize the annotation of metabolites based on their chemical formulas and neutral charges.
Some recent efforts have been made to characterize the annotation quality of plant genomes.
The functional annotation of most genes in newly sequenced genomes is inferred from similarity to previously characterized sequences, an annotation strategy that often leads to erroneous assignments.
These methods make use of available information about the annotations of genes in order to discover gene expression patterns that characterize those annotations.
After removing characterized repeats, gene annotation was performed.
The putative effect of the indel on the protein product was characterized using SeattleSeq Annotation (http://snp.gs.washington.edu/SeattleSeqAnnotation131/index.jsp) and examined for frameshift and changes in protein sequence.
The assembled BPM 24 genome was characterized for gene annotation, transcription analysis, RNA editing events, sequence variation and recombinations within the species that cause cytoplasmic male sterility in rubber tree.
