We used a caspase-3 activity indicator (C3AI), which shows fluorescence only after cleavage by caspase-3 like proteases, to further characterize whether OPT-induced cell death is correlated with increased caspase-3 activity.
To further characterize the effect of OPT, we stained MCF10A and MDA-MB231 cells with Annexin-V and PI.
Finding no effect of phenotype (data not shown), we opted to characterize this variable by the number of organ systems affected, as a plausible proxy for severity.
Although it is certainly possible that fold induction could be further increased by refinement of this scenario (e.g., by considering target chain linker lengths between 0 and 6 amino acids), optimization of these constructs was not the objective of this proof of principle investigation, and we opted to further characterize this functional architecture.
To further characterize LacZ stainings, Optical Projection Tomography (OPT) (http://www.bioptonics.com/) was used to generate 3D reconstruction and virtual sections of embryos.
Within a provider-initiated HCT strategy pre-test counselling is simplified compared with VCT, and it is commonly characterized by an opt-out approach, implying that clients who do not wish to be tested will actively have to state a lack of willingness to be tested.
To investigate the mechanisms by which OPT induces cancer cell death, we characterized the ability of OPT to induce ER stress, ROS, and autophagy.
It is important to define a new vocabulary to characterize these groups, not simply to co-opt cladistic terminology.
"I was going to say we got our butt kicked," said Ryan, who thought better (or worse) of it and opted for more colorful terms to characterize the nature of the defeat.
I've argued in detail in this Findlaw piece that it is fair to characterize both Sens. McCain and Obama as having gone back on forth on opting into public financing as their political fortunes changed.
