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High-throughput sequencing is capable of identifying thousands of loci, while providing characterizations of expression patterns and the molecular evolutionary forces acting within the venom gland.
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Such spatial and temporal characterizations of expressions paved the way for inferring regulatory networks based on spatio-temporal dynamics.
Main skills to develop and acquire: molecular biology, cloning bacterial expression of proteins and characterization of expression by SDS PAGE.
Schatz, A.R., Lee, M., Condie, R.B., Pulaski, J.T. & Kaminski, N.E. Cannabinoid receptors CB1 and CB2: a characterization of expression and adenylate cyclase modulation within the immune system.
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Tritos NA, Elmquist JK, Mastaitis JW, Flier JS, Maratos-Flier E. Characterization of expression of hypothalamic appetite-regulating peptides in obese hyperleptinemic brown adipose tissue-deficient (uncoupling protein-promoter-driven diphtheria toxin A) mice.
Here we discuss how the recombinant adeno-associated virus (rAAV) can be engineered for use in neurobehavioral studies, techniques for site-specific delivery of vector into the brain, characterization of expression profiles, and biosafety issues.
Tritos NA, Elmquist JK, W Mastaitis J, Flier JS, Maratos-Flier E. Characterization of Expression of Hypothalamic Appetite-Regulating Peptides in Obese Hyperleptinemic Brown Adipose TissUncouplingnt (UncouProtein-Promoter-DrivenDiphtheriahToxinA Toxin A) Mice* *This work was supported by a grant from Eli Lilly & Co. (to E.M.F.), NIH Grant DK-R37-28082 (to J.S.F.), and NIH Grant MH-56537 (to J.K.E.).
Upregulation of the voltage-gated sodium channel beta2 subunit in neuropathic pain models: characterization of expression in injured and non-injured primary sensory neurons.
Systematic characterization of expression changes in biological pathways among different types of cancer will eventually lead to a better understanding of which perturbations in the cell give rise to cancer.
Detailed characterization of expression kinetics and regulation are required to fully understand the β-myrcene-stimulon.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com