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Characterization of the function, regulation and metal-specificity of metal transporters is one of the basic steps needed for the understanding of transport and accumulation of toxic metals and metalloids by plants.
The accurate identification of cavities that can bind ligands on the surface of proteins is of major importance for the characterization of the function of proteins based on its structure.
The production of stable cell lines is an important technique in cell biology, and it is often the rate-limiting step in studies involving the characterization of the function of novel genes or gene mutations.
Here we report the cloning of the iterative type I PKS gene chlB1 from the chlorothricin (CHL) producer Streptomyces antibioticus DSM 40725 by a rapid PCR approach, and characterization of the function of the gene product as a 6-methylsalicyclic acid synthase (6-MSAS).
We believe that this study may pave the way for further characterization of the function played by these two genes during reproduction and uncover interactions unknown at the present time.
Although characterization of the function of these genes could provide insight into the evolution of insect development or potentially reveal novel strategies for vector control, extremely little is known about the genetic regulation of mosquito development [3], [4].
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Thus, the OC effectively forms the foundation of all functional annotation of sequenced genomes given that experimental characterization of the functions of any sizable fraction of the genes in the numerous sequenced genomes remains unrealistic for the foreseeable future.
From this the characterization of the functions in (6.2) as the only extremals in the Sobolev inequality immediately follows.
Section 2 is dedicated to the proof of Theorem 1, which contains also an explicit characterization of the functions u h, l 's and v l 's, and to the proof of Corollary 2.
However if (p>1, pnot =2) the characterization of the functions of the form (6.2) as the only ones attaining equality in (6.1) was shown much later by Cordero-Erausquin et al. [49] with a proof of the Sobolev inequality (6.1) via mass transportation in the same spirit of Gromov's proof of the isoperimetric inequality that we have seen in Sect.
Collectively, our findings provide a basis and platform for further characterization of the functions and mechanisms of coronavirus proteins.
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