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Figure 2 Characterization of macrophages.
Molecular characterization of macrophages reveals distinct types during tumorigenesis [Also see Report by Franklin et al.].
In this review, we summarize methodology for the isolation and phenotypic characterization of macrophages and DCs from the mouse intestine and discuss how this may be useful for gaining insight into the mechanisms by which mucosal immune tolerance is maintained.
Downregulation of the pro-inflammatory macrophage phenotype has been associated with the therapeutic use of bioscaffolds composed of extracellular matrix (ECM), but phenotypic characterization of macrophages has typically been limited to small number of non-specific cell surface markers or expressed proteins.
Phenotypic characterization of macrophages was performed using double immunohistochemical staining to enable evaluation of tumor cells.
In addition, this molecular characterization of macrophages in zebrafish reveals the conservation of macrophage subtypes between zebrafish and human.
However, other data, obtained by characterization of macrophage activation during disease progression, also suggest phagocytic removal of debris as the primary role of macrophages in the PNS emphasizing their non-detrimental function [33].
10.7554/eLife.07288.010 Figure 3. Isolation and molecular characterization of macrophage phenotypes.
These studies have added knowledge about the mechanisms of action of PLA2s isolated from B. atrox, contributing to better characterization of macrophage function.
Therefore, in the last years characterization of macrophage phenotype in vivo during tissue repair has been a matter of active investigation.
Characterization of macrophage-like cells to indicate inflammatory effects is important, since these cells play important role in inflammatory and immune responses and also responsible for various immune functions such as secretion of inflammatory mediators.
