Phenotypic characterization of loss-of-channel mutants does not allow deducing an explicit role of CNGC19 and CNGC20 during the nonselective uptake of Na+ during salt stress.
Assessment of the evidence suggesting that depletion of miR-2 and miR-13 group miRNAs results in embryonic apoptosis, should await functional characterization of loss-of-function mutants that remove their functions in vivo.
Further functional analysis, including re-sequencing, characterization of loss-of-function alleles and conducting gene complementation either by crossing or genetic transformation, are required to prove the role of selected possible candidate genes in coumarins biosynthesis and their regulation.
The isolation and characterization of loss-of-function (LOF) mutations in the ethylene receptors made it clear that there was a substantial level of functional overlap among members of the ethylene receptor family (Hua and Meyerowitz 1998).
The phenotypic characterization of loss-of-function mutants has implicated CNGCs to be involved in a wide range of plant processes [ 14] including tolerance to heavy metals [ 7, 19], sensitivity to various cation stresses [ 17, 20], plant development and germination [ 17, 20], programmed cell death, and disease resistance [ 11, 21].
For the sake of a more subtle characterization of packet loss processes, Clark [5] proposed a dedicated packet loss model that discriminates between isolated and bursty loss instances.
The previously defined metrics for the characterization of packet loss burstiness explicitly (resp. implicitly) consider the nominal average length of sustained loss instances (resp. inter-loss durations).
Ms. McGuire added, however, that the agency's characterization of the loss of storage space as de minimis is not necessarily set in stone but could be subject to interpretation or modification by the agency itself or the courts.
