Main skills to develop and acquire: molecular biology, cloning bacterial expression of proteins and characterization of expression by SDS PAGE.
Schatz, A.R., Lee, M., Condie, R.B., Pulaski, J.T. & Kaminski, N.E. Cannabinoid receptors CB1 and CB2: a characterization of expression and adenylate cyclase modulation within the immune system.
Moskovitz, J., Malik, A., Hernandez, A., Band, M. & Avivi, A. Methionine sulfoxide reductases and methionine sulfoxide in the subterranean mole rat (Spalax): characterization of expression under various oxygen conditions.
Tritos NA, Elmquist JK, Mastaitis JW, Flier JS, Maratos-Flier E. Characterization of expression of hypothalamic appetite-regulating peptides in obese hyperleptinemic brown adipose tissue-deficient (uncoupling protein-promoter-driven diphtheria toxin A) mice.
Here we discuss how the recombinant adeno-associated virus (rAAV) can be engineered for use in neurobehavioral studies, techniques for site-specific delivery of vector into the brain, characterization of expression profiles, and biosafety issues.
Tritos NA, Elmquist JK, W Mastaitis J, Flier JS, Maratos-Flier E. Characterization of Expression of Hypothalamic Appetite-Regulating Peptides in Obese Hyperleptinemic Brown Adipose TissUncouplingnt (UncouProtein-Promoter-DrivenDiphtheriahToxinA Toxin A) Mice* *This work was supported by a grant from Eli Lilly & Co. (to E.M.F.), NIH Grant DK-R37-28082 (to J.S.F.), and NIH Grant MH-56537 (to J.K.E.).
High-throughput sequencing is capable of identifying thousands of loci, while providing characterizations of expression patterns and the molecular evolutionary forces acting within the venom gland.
The estimated parameters were easy to interpret, and aided in the characterization of gene expression and co-expression profiles.
The integration of gene expression information from multiple microarray studies can enhance the characterization of gene expression profiles that are consistently expressed across experiments.
In concordance with the characterization of StSN1 expression in potato [ 3], the expression pattern of MsSN1 suggests that SN1 is a component of the constitutive defense barrier.
Such spatial and temporal characterizations of expressions paved the way for inferring regulatory networks based on spatio-temporal dynamics.
