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An indispensable part of the work of genebanks is the phenotypic characterization of accessions (19).
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Data on agro-morphological characterizations of accessions in Musa collections are published in the Musa Germplasm Information System (IPGRI-INIBAP(Bioversity), 2003).
In addition, the genetic characterization of these accessions organized the spring wheat core collection into seven subpopulations of genetically related accessions.
Thus, the scientific description and characterization of the accessions in Ghana was imperative.
Based on the results of a blind test, the genotyping system was confirmed to be suitable for characterization of unknown accessions.
In order to verify the reliability of the optimized genotyping platform and its potential as a standardized methodology for molecular characterization of new accessions, a set of anonymous samples was analysed [ Additional Information 3].
In this study, we attempted to develop an optimized SSR-based system for molecular characterization of Musa accessions that could be used as the basis for the foundation of the Musa Genotyping Centre (MGC).
For a comprehensive molecular characterization of both accessions, the metabolome, proteome and the phosphoproteome, i.e. phosphopeptide abundance, was analysed applying an integrative analytical GC-MS and LC-MS platform [ 38– 438.
Yet, such systems would enable characterization of unknown accessions as well as hybrid breeding materials and cultivars, and assist greatly in early characterization of all primary recombinants that have to be generated in large numbers whenever high precision of alien introgression is required [ 45].
Over the past few years, proposals of methodologies for establishing core collections that use data generated by the application of molecular markers in conjunction with the morpho-agronomical characterization of the accessions have been given more attention [ 10, 12, 16, 17].
We have recently published a report on the cloning, expression and characterization of CotA (accession no. YP_077905), a new Bacillus licheniformis laccase, in E. coli [ 35].
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