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Figure 2 Characterization of PMNPs.
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Figure 3 Characterization of MNCs fabricated from three PMNPs.
TEM image of PMNPs is demonstrated in Fig. 3B.
The concentration of PMNPs was set at 1.0 mg/mL.
Open image in new window Fig. 3 A ζ-potentials of Fe3O4 MNPs, Fe3O4/SiO2, Fe3O4/SiO2/APS, PMNPs (COOH), and PMNPs (OH), B TEM image of PMNPs, C photograph showing the separation of PMNPs from the solvent.
Finally, three samples of PMNPs were successfully obtained from the precipitates [25, 26], each coated with different oleic acid amounts: 19 (low PMNPs, LMNPs), 33 (medium PMNPs, MMNPs), and 46 (high PMNPs, HMNPs) wt.% (Figure 2b).
For simple sugar enrichment and analysis, glucose was chosen as standard to evaluate the selectivity of PMNPs.
The derivative weight curve of an iron-oleate precursor used for MNP synthesis also agreed with the derivative peaks of PMNPs (Additional file 1: Figure S4).
For complex saccharides samples enrichment and analysis, N-glycans from ovalbumin were chosen as standard to evaluate the selectivity of PMNPs.
To investigate the effect of primary ligand on MNCs, the interactions of oleic acid molecules on the surface of MNPs were analyzed through derivative weight curves of the three samples of PMNPs (Figure 2c).
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