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The monoclonal antibodies against native PCX and recombinant PCX were further characterised using the following methods.
AD-MSC were characterised using the following accepted criteria [ 23]: adherence to plastic; osteogenic and chondrogenic differentiation as evidence of multipotent differentiation potential; and specific surface antigen expression.
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Patients were identified and characterised using the Ward Watcher database.
Tumour antigen-presenting cells were phenotypically characterised by flow cytometry using the following monoclonal antibodies (mAbs): HLA-ABC, HLA-DRDQDP, CD80 and CD83 conjugated with fluorescein isothiocyanate (FITC; eBioscience, San Diego, CA, USA), CD86-FITC (BD PharMingen, San Jose, CA, USA) and anti-CD11c conjugated with phycoerythrin (PE; eBioscience).
Monocyte human leucocyte antigen-DR (mHLA-DR) membrane density (geometric mean fluorescent intensity) was characterised using flow cytometry following a 20-hour incubation.
The AAPC was calculated using the following formula: It may not always be true to expect that a single AAPC can accurately characterise the trend in health research productivity over an entire 15-year period between 2000 and 2014.
using the following parameters.
Avoid using the following for research.
The repeatability is characterised using nine observations of the Orion Bar.
The strains were characterised using pulsed field gel electrophoresis (PFGE).
The heterogenic populations were characterised using cell culture selection.
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