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The first step towards the annotation of a tag requires matching it to a previously characterised sequence e.g. an Expressed Sequence Tag (EST) or genomic sequence.
Several studies that examined chimpanzee and human genomes comparatively, located and characterised sequence differences, including single-base pair indels, monomeric and multi-base pair extensions (repeats), indels with random DNA sequences, and transposon insertions [ 5, 10, 15]; and more are currently under way.
The domain structure of EYS has been predicted from the characterised sequence of EYS [ Abd et-aliz et al., 2008; Collin et al., 2008] as having several EGF-like and Laminin G domains.
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Ten faecal microbiota samples were detected by Illumina Miseq sequencing of the 16S rRNA genes from 367205 characterised sequences.
Using these additional datasets we characterised sequences in the spheroid body's genome for recA, recF, psbC and psbD.
Here, we characterised sequences of 2nd exon of the MHC class I and class II genes in the Scandinavian population of brown bear Ursus arctos.
Previously characterised sequences from chicken and ostrich (Struthio camelus) designated as MSMB [ 15, 28] correspond to MSMB1, while the gene currently annotated as MSMB by the NCBI "Gene" database http://www.ncbi.nlm.nih.gov/gene?term=msmb%20gallus corresponds to MSMB2.
This made it possible to characterise sequence mismatches that affect microarray hybridisation on a genome-wide scale.
In order to characterise sequence differences in detail, multiple sequence alignments (MSAs) were generated on the basis of a representative selection of TrpA and TrpB sequences.
Insert DNA was characterised by sequence determination (ABI 3700 DNA sequencer) and subsequent comparison to the public database (BLASTn, BLASTx).
In fact, examination of their data reveals that the cDNA is derived from the previously characterised SIRT7 sequence (Frye, 2000).
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