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We raised and characterised N- and C-terminal Setd2 antibodies to confirm that Setd2 protein levels were specifically diminished as a result of its mRNA knockdown.
The ability to perform bacterial N-glycosylation of target proteins in E. coli has been demonstrated using the well characterised N-glycosylation pathway from Campylobacter jejuni [5 7].
The focus here has been on the conserved functional core of PPP1R15, but there remains room for further modulation of both isoforms by their large, poorly characterised N-terminal regions.
This paper uses APSIM scenario modelling to characterise N uptake patterns of sugarcane plant and ratoon crops in response to seasonal variability and different climatic and management conditions (e.g. level of water and N input, time of planting/ratooning) at five sites within the Australian sugarcane growing region.
To characterise N gonorrhoeae, C trachomatis and M genitalium samples from Guinea-Bissau and to define bacterial populations, possible transmission chains and for N gonorrhoeae spread of antibiotic-resistant isolates.
Another metric set used in texture analysis is Minkowski functionals, a family of statistical metrics able to characterise n-dimensional space geometrically and topologically (Arns et al., 2010).
Each speaker k is characterised by N k the number of voiced segments.
As both of the glycoproteins identified are characterised by N- and O-linked carbohydrate-rich extracellular regions [28], [29], we sought to identify specific glycans that have high binding affinity for SSL5.
These are characterised by N-terminal PYD and C-terminal HIN-200 (hemopoetic interferon-inducible nuclear antigen with 200 repeats) DNA binding domains.
They are all structurally characterised by N-terminal pleckstrin-homology (PH) and phosphotyrosine-binding (PTB) domains, which are required for coupling to the activated insulin/IGF receptors, and a C-terminal region with multiple sites for tyrosine phosphorylation by the receptors.
The class membership probabilities could then be assigned using eq 2. Given n c classes, a fragment is characterised by an n c -dimensional vector, so a protein can be seen as n r - k + 1 vectors in an n c -dimensional space.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com