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We generated a list of 41 genes, of which 35 are well characterised cell cycle genes according to current Gene Ontology annotation.
A panel of 15 previously characterised cell lines [ 25] were infected with serial dilutions of reovirus and assessed for cell survival.
Therefore, we used bcr-abl transformed FDCP-mix p210 bcr/abl cells, a well characterised cell culture model system for CML Pierceetet al, 1998).
The aim of this study was to determine if Raman spectroscopy could be used to monitor changes in a well characterised cell population (human dental pulp stromal cells (DPSCs)) by taking repeated Raman measurements from the same cell populations in osteoinductive culture over time and under aseptic conditions.
Since our cell type specific gene signatures were derived from phenotypically well characterised cell types compared to empirical stratification based on expression data, we were also able to identify a range of myoepithelial type genes in ER-positive tumours as well as those in basal-like breast cancers.
In each case, different steps are considered in the design of the process and further characterised: cell harvesting, washing, pasteurisation and spry-drying.
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The described approach is not just a means to simultaneously characterise cell adhesion and ligand distribution with SMLM, but also illustrates that SMLM can be used to characterise biointerfaces at the molecular level46.
In this paper, we present a new approach to characterise cell growth on porous silicon gradients displaying pore sizes from several thousands to a few nanometers.
Furthermore, to characterise cell death we performed AnnexinV/7AAD staining.
Although many studies characterising cell populations arising from specific cord regions have been reported [ 7, 10– 14], there are no known single studies which have aimed to isolate, culture, and characterise cells from all cord regions from the same cord.
FITC- and PE-labelled mAbs against DC markers (BD Biosciences Pharmingen, San Jose, CA, USA) and isotype-matched labelled controls were used to characterise cell surface phenotypes by flow cytometry.
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