Pharmacological tools and knockout mice were used to characterise candidate sensory pathways identified by expression analysis.
Whilst it is unclear to what extent ancient vertebrate CNEs are composed of 'conventional', previously characterised TFBSs, candidate motif search approaches have provided evidence that mammalian UCEs are enriched for known TFBS motifs [ 26] and that ancient vertebrate CNEs associated with genes involved in CNS development show enrichment for Oct and Sox motifs [ 20].
To characterise possible candidate genes upstream of prostratin synthesis we screened the E. fischeriana transcriptome for enzymes in the TBB (KEGG map00900) and DB pathways.
In the CNS, TIMP-1 was first characterised as a candidate plasticity gene induced by seizures and by stimuli leading to long term potentiation (LTP) [3], a form of synaptic plasticity considered as a cellular substrate of learning and memory.
Overall DLC1, the most well characterised tumour suppressor candidate in 8p22, was only affected in a single cell line by a copy number decrease that included two other genes.
We further characterised the 4 candidate genes which were upregulated to the greatest extent by hypoxia/DMOG – namely ANGPTL4, EFNA3, TGF β1 and VEGF - to be hypoxia-regulated in Caco-2 through the HIF-1α isoform.
In addition, although implicated in tumour development rather than cell invasion/metastases, SLC5A8, a sodium symporter identified by a global search for genes that were aberrantly methylated at high frequency in human colon cancer and consequently repressed in expression (Li et al, 2003), was characterised as a candidate tumour suppressor.
Most studies have examined associations between a few well-characterised candidate polymorphisms and task-related brain activation differences in individual regions of interest.
Due to the severity of EBJ in this case, a very likely causative mutation in the coding region of one of the well-characterised candidate genes was hypothesised.
To better understand the molecular abnormalities in small bowel adenocarcinoma (SBA), we characterised a number of candidate oncogenic pathways and the immunophenotype of this rare cancer.
