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Despite being a well characterised assay for mapping activation and repression motifs, including those in other CP2 family members [2], [13], we were unable to detect any region of CRTR-1 with transactivation properties using this method.
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The first stage was to characterise assay performance (mean value±precision) by analysis of 16 replicates of each of three different QCs representing high-, mid- and low-range concentrations on the calibration curve.
Cells were characterised by assay of alkaline phosphatase, detection of type 1 collagen, and production of osteocalcin.
Therefore, one can begin to imagine how a large panel of cancer cell lines that have been extensively characterised and assayed for their sensitivity to a large collection of pre-clinical and clinical therapeutic agents might enable therapeutic biomarker discovery.
We have shown that under a precise restatement of the McDougal et al. assumptions, there exists a redundancy in the parameters they chose to characterise the assay.
Furthermore, formation and loss of gamma H2AX is cell line dependent, so further studies will be needed to characterise this assay fully.
The aims of the present study were to characterise an assay for cAMP-binding proteins in ovarian cancer and then to measure levels in a series of tumours with a view to developing a potential prognostic indicator for this disease.
Basically, four forms of cell locomotion could be characterised in this assay.
Reducing power is an ionic electron transfer assay characterised by the formation of Perl's Prussian blue coloration after ionic reduction, to produce a reduction in the ferric ion/ ferricyanide complex to ferrous form.
It is a well-characterised assay, however, which can improve targeting of chemoprophylaxis to those most likely to benefit.
In this study, the RmS-15 expressed in the yeast Pichia pastoris was characterised using kinetic assays and in vitro analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com