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All of the latter three proteins have been characterised as positive regulators of PI3K signalling [ 64].
Fibroblast cells were characterised as positive by immunohistochemistry staining for vimentin and smooth muscle actin and by lack of pan-cadherin staining (data not shown).
Horse sera previously characterised as positive or negative for BDV antibodies in a BDV-ELISA were used as controls, whereof one of the positive controls also was PCR-positive and positive at Western blotting [ 7].
18 During the analysis, possible answers for each question were characterised as positive (P), negative (N), or neutral (NEU) and pooled for each one of the themes (A, B and C; table 1).
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The PKC profile of 293 cells was also characterised as a positive control for the primer sets used.
20 21 The dependent variable for this analysis is HIV status, characterised as a positive or negative blood test.
Similarly, tumour samples from patients that were characterised as nodal positive (N1) harboured significantly less Hoxc8 mRNA copies than samples from N0 patients.
Indeed, taking a two-fold increase in signal over mean noise as significant (Kramer et al, 2004), this would translate into an increase of >28% for M30 and >26% for M65 being characterised as a positive result.
These filters were defined in a recent paper [13] that highlights the need to remove a variety of classes of compound that are likely to be characterised as false positives in biochemical screening.
Besides, for ALCAM, clinical specimen were characterised as membranous-positive or as non-membranous staining.
However, the elderly lady is also presented in a context of material security and emotional well-being, while the body stance of the nun, bending forward from behind the armchair to lend a hand in opening the parcel, can be characterised as caring, triggering positive judgement values.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com