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Furthermore, that UEA and PNA staining is a useful tool in the characterisation of cells cultured on biomaterials, therefore helpful in identifying biomaterials that are suitable for specific tissue engineering purposes.
During cell culture, in situ imaging and morphological characterisation of cells was assessed using brightfield light and/or fluorescence microscopy, and later confirmed by staining of fixed cells using immunofluorescence microscopy.
The results of this study underline that in context of hESC it is beneficial to have detailed characterisation of cells at the single cell level whenever the cells have been manipulated.
CK participated in the design and coordination of the study, and performed the characterisation of cells.
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Here we describe the development and preliminary characterisation of cell lines from a diverse range of tissues from Pteropus alecto, the black flying fox.
Characterisation of cell culture media by 1H-NMR has facilitated the detailed scrutiny of metabolites involved in a variety of biochemical pathways [28], [29], [30] and most recently, has been used to examine media conditions associated with human hepatoma cell growth in vitro [31] and for potential tissue engineering applications [32].
SZ performed part of the inhibitory studies and characterisation of cell lines.
Flow cytometry characterisation of cell subsets in SVF is shown in table 1.
The detailed characterisation of cell line phenotypes will support the selection of models to use for specific preclinical investigations.
These lines provided good markers for the characterisation of cell proliferation and differentiation during development of the outer integument.
These modifications therefore represent significant improvements to previous works and allow careful characterisation of cell-surface events.
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