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We have examined the character of sequence conservation in the mammalian inflammatory region and identified clade-specific elements.
S[ x] indicates the xth character of sequence S, n c represents the number of amino acids used to predict a cleavage site, and i c, j c denote the start of the segments used to predict the cleavage likelihoods at site c i and c j, respectively.
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The phylogenetic tree is represented by a matrix A in which a ij is the state of character j in sequence i, and the ith row is the character vector of sequence i.
Consistent with the ancestral exonic character of the sequence, coding frame and amino acid sequence encoded were highly conserved across species within these regions, and all new introns were a multiple of 3 nts, thus conserving coding frame.
However, to verify the repetitive character of each sequence motif we performed BLAST search against available B. vulgaris sequences.
Due to the basic character of DNA sequence, AS1411, DTNs, and DTNs-AS1411 can assemble automatically by base pair complementary mechanism at suitable temperature.
As shown in Figure 3B, a further substitution (I21S, mutation number 5) is able to compensate for the negative impact of K6N+F11V+P20L, restoring almost completely the SP character of the sequence.
In the region, ranging from nucleotides 711260 716600, 11 hits are obtained, clearly indicating the repeat character of this sequence which has also been described by Gomez-Valero et al. [ 11].
Similarity scores are a more reliable measure of the native-like character of designed sequences, because they take into account the diversity of the natural sequences [42], [67].
This might be due to the partial character of these sequences, which were used from a previous study [ 29].
Further, inversions must have contributed to the basic character of DNA sequences since the origin of life.
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