To demonstrate that a pore-less TRPML3 protein does not function as an ion channel, we generated cDNA encoding a fusion protein of TRPML3(Δ exon11) with yellow fluorescent protein and expressed the mutant channel protein in HEK293 cells.
Using a microfluidic T-channel, we generate a liquid interface by co-flowing two different electrolyte streams side-by-side.
In an attempt to identify TRPA1 mutants that have specific deficits in sensitivity to reactive chemicals (NO donors and/or IA) but not non-reactive agonists (i.e., with little/no general defects in overall channel function), we generated the three described individual cysteine mutants of TRPA1.
To test whether the serine side chain determines the single-channel conductance, we generated heteropentamers of GLIC wt and S6′G mutant (S/G6′-heteropentamers) by co-injecting oocytes or co-transfecting BHK cells with mixtures of DNAs coding for wt and S6′G subunits, defined by the proportion (a) of mutated DNA versus total DNA.
To study the function of the channel in Drosophila, we generated subdued knockout strains.
Since EC3 loop contains a motif similar to ion channel selectivity filter, we generated an alignment guided by presence of similar motifs to generate Model 3 (using alignment in Fig. S2C).
To examine the functional significance of Kv4 channels in Drosophila neurons, we generated transgenic lines expressing a dominant-negative Kv4 channel subunit, DNKv4.
In order to investigate the effect of channel memory for a given ADIR filter memory on the performance of the proposed method, we generated channels with longer impulse responses than that of h a (n), nevertheless, we made sure that these channels have exactly the same spectral characteristics or eigenvalue spread with h a (n).
To capture channel activity, we generate and analyze 802.11b/g packets using the multi-radio mesh nodes in our testbed BilMesh, which is a multi-hop, multi-radio wireless mesh networking testbed we have established at Bilkent University.
Since GIRK2 is the only subunit that assembles into functional homo-tetrameric channels (Luscher & Slesinger, 2010), we generated transgenic mice expressing a FLAG-GIRK2 construct under the regulatory elements of the sodium channel Nav1.8 to drive selective expression in peripheral sensory neurons (Shields et al, 2012).
