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However, little is known about how these signaling systems impact the kcnq channels in zebrafish sensory pathways.
Our identification of Kcnq channels in zebrafish may offer a new in vivo model system for screening KCNQ channel modulators/drugs and their effects on regeneration.
Although the description and function of ion channels in zebrafish systems biology is beginning to emerge [ 7- 11], little is known about the expression and functional characteristics of most zebrafish ion channels.
By expressing light-gated ion channels in zebrafish, the pacemaker or sino-atrial node was recently located at the inner curvature of the venous pole, the region that is added last to the cardiac tube.
Mouse anti-pan-sodium channel antibody (1 200, Sigma-Aldrich monoclonal antibody from Clone K58/35, raised to sequence conserved between all vertebrates and shown to be specific to sodium channels in zebrafish by co-localisation with other nodal proteins and complementary expression to internodal markers [ 37]).
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These findings suggest that sleep is regulated by neuronal-circuit specific potassium chaneuronal-circuit specific, and mammals.
Identification of kcnqx potassium channel transcripts in zebrafish (Danio rerio) remains to be fully characterized although some genes have been mapped to the genome.
We identified a member of the Shal subfamily of K+ channel subunits in Zebrafish and named it zShal3, since it appears to be the ortholog of the mammalian Kv4.3 subunit.
In the present study we demonstrate the first cloning, localization, and functional expression of two Na+ channel β1 orthologs in zebrafish, scn1ba_tv1 and scn1ba_tv2, which arise from alternative splicing of scn1ba.
Because the acetylcholine receptor channel is well characterized in zebrafish and is abundant as skeletal muscle is found throughout the body, we sought to knock down its expression as a general test of the feasibility of this approach.
The identification of conserved orthologs to all 4 voltage-gated sodium channel beta subunit genes in zebrafish and the lack of evidence for beta subunit genes in invertebrate chordates together indicate that this gene family emerged early in vertebrate evolution, prior to the divergence of teleosts and tetrapods.
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