Sentence examples for changing the differentiation from inspiring English sources

The same amount of TNF-α was added every 24 hours without changing the differentiation medium.

After induction towards one of these lineages, they retain the ability to be committed to the other one by changing the differentiation medium and cocktail for a limited time.

The results however were not additive, indicating that removing OA function or changing the sexual differentiation of the FruM OA neurons had similar effects on the modulation of male courtship object choice.

When C2C12 cultures reached 70 90 % confluency they were differentiated by changing the media to differentiation medium consisting of DMEM supplemented with 2 % horse serum, 200 U/ml penicillin and 200 µg/ml streptomycin.

This reduction was explained by the suppression of immune reactions, especially by changing the function or differentiation of DC and regulatory T-cells [ 29].

Since cells had attached sufficiently during the expansion culture period in the same plate wells prior to day 0 in our experiment, these extraordinary morphological changes are likely a response to changing the culture environment to differentiation medium.

C2 mouse myotube cultures were prepared by growing myoblasts (purchased from ATCC, Manassas, VA, USA) on glass coverslips or in culture dishes in DMEM containing 20% fetal bovine serum (growth medium) until confluence and then inducing differentiation by changing the medium to DMEM containing 2% horse serum (differentiation medium); fresh differentiation medium was added each day for 4 5 d [53].

Breaking this symmetry changes the parameter combinations that generate heterogeneous differentiation without changing the bifurcation diagram (Additional file 5: Figure S4).

Therefore, an increase in IL-6-producing Ag-presenting DC could lower the affinity threshold for Tfh-cell differentiation without changing the overall affinity threshold for clonal selection of the whole Ag-specific CD4+ T-cell repertoire.

A general approach has been to try to change the phenotype of various cell types in vitro by changing the environment and adding growth and differentiation factors.

Differentiation was initiated 3 days after re-plating on day of differentiation (DoD) 0 by changing the medium to knockout serum replacement medium (KSR) (Knockout DMEM with 15%% knockout serum replacement, 2 mM Glutamax, 0.1 mM MEM non-essential amino acids and 50 μM beta-mercaptoethanol) supplemented with 35 ng/ml noggin, 600 nM dorsomorphin and 10 μM SB-431642.