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Alternatively, proliferating cells C17.2 cells were seeded in 6-well plates at a density of 2000 cells/cm2 in complete DMEM medium and the medium was carefully removed the following day and the cells were exposed to differentiation medium containing 50 µg/mL CeO2 or 50 µg/mL Sm- CeO2 for 7 days, changing half of the differentiation medium at day 3 and at day 6.
Cultures were fed by changing half of the medium every 3 days.
We restarted ECIS impedance recording, changing half the medium at 36, 96 and 120 h.
Cells were fed by changing half of the medium every 3 days.
The cells were subsequently cultured at 37°C in 5% CO2 changing half of the medium every 2 3 days.
Although changing half the medium could have affected impedance data for a short time, each group tended to persist in its own manner (2 4, Figure 4B).
Similar(52)
Changing half-life and potency together yielded a marked (31-fold) increase in forgiveness.
Other possibilities raised were locally raising NICD levels or changing half-life by removing the PEST domain.
For this we will focus on other strategies to fine tune the system, such as mutating operator sequences and changing half-lifes.
Then, we confirmed the changing half-life of the mutant pVHLs using cycloheximide (CHX -mediated pulse CHX -mediatedn both HEK293T (Fig. 1b) and 786-O cells (Fig. 1c).
This value from the simulations was also similar to the value from the product of the two independent effects, changing half-life RF = 8.57 and changing potency RF = 3.63.
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