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Relevant expression changes were validated by qPCR experiments.
These changes were validated by quantitative RT-PCR (Fig. 4D).
Much larger gene changes were validated in a number of novel genes as exemplified by TMEM176A, which showed 35-fold enrichment in the cortex and 30-fold enrichment in hippocampus of FRL animals relative to FSL.
Proteome changes were validated by western blot analysis, which confirmed that microtubule-associated protein, RP/EB family, member 1 (Mapre1; GeneID: 13589), eukaryotic translation elongation factor 2 (EF-2; GeneID: 13629) (Figure 5A) and heat shock protein 70 kDa (Hsp70; GeneID: 15511) (Figure 5B) increase following LT challenge.
These changes were validated by orthogonal immunoblotting.
Gene expression changes were validated by RT-PCR.
Similar(43)
The ability of the FE models to simulate the moisture diffusion and suction variations in relation to climate changes is validated through two case studies involving field observations.
The effect of reading on VIQ changes was validated by structural brain changes.
Can these changes be validated by other experimental approaches like RT-PCR?
A significant proportion of ERα associated gene expression changes was validated by this cross-platform comparison.
Its role in the understanding of evolutionary changes was validated by determining the association between the commonly co-expressed genes and the dN/dS value, which is a well-known parameter of molecular evolution rate.
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