In spinner flask PI cultures, half-volume medium changes were performed every 2 3 days.
Design changes were performed by means of finite element analyses.
Both upward and downward temperature changes were performed with temperature combinations of 350/500 and 500/350 °C.
Significant changes were performed in the apparatus, with the design of a new measuring cell and the simplification of the tubing system.
Animal monitoring and cage changes were performed weekly by the experimenter or a professional animal caretaker.
Further investigations of the structural changes were performed using SEM, TEM, and particle size analyses.
Intergroup comparison at the initial stage and of the treatment changes were performed by analysis of variance.
In contrast to the previous attempts, the temperature changes were performed in a continuous manner at a rate of 1°C/min.
Descriptive statistics for the pretreatment variables for both groups, as well as descriptive and inferential statistics to assess the intra- and intergroup differences in vertical dimension changes, were performed.
An alternate set list with minor changes were performed after the first North American leg of the tour.
Partial medium changes were performed every 5 days.
