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Most of the low-fold gene changes we verified from the IPA networks involved genes critical to the regulation of cholesterol and steroid synthesis in immune cells and in macrophages in particular.
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In agreement with the hypothesis that the two species did not undergo notable changes [ 17], we verified that the overall structure of both co-expression networks are scale-free and have comparable properties.
Dinc et al. [ 8] suggested the PDW as a new index in the diagnosis of acute appendicitis, and we verified changes in the PDW in patients with AGA.
Microarray data were validated by rigorous statistical tests; however, we verified changes by RT-qPCR of TIMP-1 and PLZF, two genes expressed abnormally in other cancers [ 44, 45].
Table 3 lists genes up- or down-regulated >2-fold in ofd1 MO-injected embryos (see Supplementary Material, Table S1 for the complete set) and we verified changed expression of selected genes by real-time RT PCR (Fig. 7).
Concomitant with this composition change, we also verified a modification in the sophistication level on the intra-bloc exports.
Because gene microarray technology platforms change, we also verified candidate gene differences between C57BL/6J and DBA/2J using a newer microarray data set, based on the Affymetrix M430 A and B chips.
As a preliminary step, we verified significant changes in the expression level of Fabp7 in the COUP-TFI−/− MEF cell line by real-time RT-PCR (Figure 3A).
We verified the changes observed in lipid metabolism proteins by measuring Apo-AI and Apo-B concentrations (representing HDL-cholesterol and LDL-cholesterol respectively) by ELISA in a larger group of samples collected from malaria-infected and uninfected primigravidae and multigravidae with and without SA (primigravidae n = 150; multigravidae n = 145).
We verified that changes in mRNA expression in KO BAT correlated with protein levels.
In contrast, in our present study we verified no changes in VEGF secretion of KC keratocytes following CXL.
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