Sentence examples for changes we constructed from inspiring English sources

However, because these mutants had multiple changes, we constructed two 39.2 mutant clones by site-directed mutagenesis of plasmid pAC, carrying the minimal 39.2T4 gene.

To gain structural insight into the possible effects of the cnaB mutational changes, we constructed a homology model of the regulatory subunit of calcineurin.

Our conclusion does not change when we constructed the PMP vector by assigning the value of 0 to sperm and cancer cells and the value of 1 to normal somatic cells.

In order to test the functional effect of the human specific amino acids changes, we first constructed two mutant hMCPH1s by introducing a backward mutation of changing the human-specific amino acid to an ancestral amino acid (Met96Thr) and introducing a random mutation (Met96Leu) at Site-96.

To attempt to determine the basic structure required for a conformational change in MENT, we constructed mononucleosomes and found that this was the necessary structure required for a conformational change in the molecule.

For the representation of the isochoric change of state, we constructed (Δu/v) versus x1 diagram with 1 × 105 Pa reference as a basis for LNG, LPG and R13a.

(A ) For each of the 166 recurrently mutated genes associated with a change in MIC, we constructed a patient-by-gene binary vector.

To obtain a comprehensive view of how VPA treatment might change chromatin structure, we constructed DHSS libraries from VPA- (200 mg/kg, i.p).

Next, to evaluate monoallelic changes in expression of DLGAP4 isoform b due to the allele-specific changes in DNA methylation, we constructed mouse-human E2 cell hybrids containing only a single derivative (der8 or der20) or normal (chr20 or chr8) human chromosome derived from translocation carriers (Supplementary Material, Fig. S4B and D) (33).

To test whether transcription was responsible for the observed changes in colony morphology, we constructed JM83Δhns and JM83ΔstpA::cat mutants defective in chromosomal structuring proteins H-NS and StpA, respectively.

To simulate methylation profile changes with realistic statistics, we constructed our simulation from a real RRBS dataset.