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Results: Comparing the TGF-β1 siRNA 0.25 mg/kg treatment group to the model group, the TGF-β1 siRNA negative control group and the TGF-β1 siRNA 0.125 mg/kg treatment group showed significantly reduced levels of pathological changes, protein expression and the mRNA expression of TGF-β1 TGF-β1I collagen and type III collagen (P < 0.01).
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Polymorphisms in regulatory regions can change protein expression and may be associated with susceptibility to certain diseases [27].
Our results suggest that human skin might respond to RF-EMF and change protein expression profile.
An online database [ 31] was used to assess relevance of significantly changed protein expressions to relapse-free survival.
Changes in protein expression are expressed as a percent of the control values.
To designate significant changes in protein expression, fold-changes >1.5 or <0.66 were set as cutoff values.
Changes in protein expression lag behind RNA changes.
ID3 showed dramatic changes of protein expression, closely following the changes of transcript levels.
As a result, insights into the global changes in protein expression based on changing environmental conditions were limited.
The apparent changes in three of the proteins were confirmed by semi-quantative PCR, showing that the changes in mRNA expression were reflected in changes in protein expression.
To determine if the changes in gene expression were representative of changes in protein expression, we examined ERα protein levels.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com