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The time-dependent changes of viability in control and LHON cybrids, preincubated with 12 mg/ml of 2,5-HD reported in figures 1C and 1D, showed a similar behaviour.
The time-dependent changes of viability in control and LHON fibroblasts treated with 12 mg/ml of 2,5-HD (figure 3C and 3D) reveals that, consistently with the other experiments, the 11778/ND4 and 14484/ND6 mutations associated with haplogroup J1 were the most sensitive.
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While Fe-SP at concentrations between 300 nM and 1 µM proved to be highly cytotoxic to SKOV-3 and OVCAR-3 cells, treatment of HeLa cells or primary fibroblasts with 1 µM Fe-SP did not result in any change of viability.
RECIST cannot accurately reflect the change of viability of a tumour cell.
The data (in fluorescence units from the microplate reader) for the test wells were normalized to the assay control (DMEM only), and bystander effects were calculated as a change of viability in the irradiated group compared to the unirradiated group per sample.
Additionally, in our study, although IGF-1 treatment alone could promote cell viability, there were no significant changes of cell viability during AMP-induced cell death in both breast cancer cells (data not shown).
Compared to the NP-free control, all four cell lines exposed to TiO2 NPs showed cytotoxicity in a dosage-dependent and time-dependent manner, which was associated with the changes of cell viability and cell morphology, increased intercellular ROS levels, and decreased intracellular GSH levels.
Changes of the viability of cBMSCs cultured in different extracts were assessed through MTT assay.
Phenotypes were clustered according to log2 fold changes of cell viability.
The concentration dependent changes of cell viability in ebselen treated MM cells, determined by CCK-8 Kit, are shown in Figure 1.
For evaluation of the effects of changes in viability of the brain slices during the incubation, aECF samples can be taken at different time points (after 1, 2, 3, etc. hours).
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