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Nevertheless, chemical changes of substrates and metabolic activity can be made visible via fluorophores, and tracked both inside or around cells for advanced image and video analysis of soil organisms, cell growth, movement and substrate usage.
The metabolic stories may be ranked in different ways depending on which interpretation one wishes to emphasize for the causal link between two affected metabolites: enzyme activation, enzyme inhibition or domino effect on the concentration changes of substrates and products.
These scenarios may then be ranked in different ways depending on which interpretation one wishes to emphasize for the causal link between two affected metabolites: enzyme activation, enzyme inhibition or domino effect on the concentration changes of substrates and products.
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It consists of four parts: extracellular environment and constraints setting mode, table for optimal metabolic flux distribution and chart for changes of substrate concentration, dynamic flux distribution viewer and dynamic hierarchical regulatory network viewer.
Substitution of Val for I74 and replacement of F91 with His or Tyr both resulted in small structure changes of substrate binding pocket, which contributed to improve protein stability and catalytic efficiency.
Pulse feeding caused dramatic changes of substrate concentration and pH values in a cycle of SBR operation (Fig. 2), and enhanced substrate penetration into sludge aggregates and substrate storage at the beginning of each cycle.
Taken together, these findings suggest that under static conditions matrix rigidity acts as a tactile set-point to regulate cell traction forces, but dynamic responses to stretch are governed by mechanisms that appear to be invariant with regard to changes of substrate stiffness.
Together these findings suggest that there is a gender-specific response with respect to changes of substrate utilization following sustained consumption of fructose.
After binding to its substrates, Pin1 can facilitate the cis-trans isomerization of pSP/pTP peptidyl-prolyl bonds through its PPIase domain, resulting in conformational and functional changes of substrate proteins.
The motion on the fast time-scale is dominated by the complex C decoupled from the substrate S. On the slow part, the changes of substrate and complex are balanced.
In summary the proteolytic potential of the gut microbiota appeared to be enhanced upon ageing, even if there are changes of substrate, and a clear loss of genes involved in the metabolism of carbohydrates in the aged-type microbiota was observed.
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