The amino acid substitutions resulting in a partial change of specificity were identified and combined into one superior variant designated NN1.
Mutating the tyrosine to an asparagine in ELF4-DBD led to a clear change of specificity towards that of class III both at +4 and +5.
In contrast, mutation of a class III-specific glutamate that contacts water molecule between positions −1 and 1 (Kodandapani et al, 1996) to a glutamine residue led to a clear change of specificity of ELF4 towards class III at positions −1 and −2.
The hypothesis tested in this article is that NORK evolution has been driven by changes of rhizobial specificity (i.e. acquisition of a new partner, or co-speciation of partners).
Second, we examined the top-scoring site pairs, and compared them with known SDRs from a mutagenesis study [ 13] in which ten sites of the ERBB2IP-1 domain were mutated and the corresponding changes of peptide specificity reported.
It would be interesting to study changes of coreceptor specificities due to the insertion of argine and lysine residues in the V3 loop of CCR5-dependent viruses.
Further, the functional consequences of changes in specificity of immunological recognition can often be measured in exquisite detail.
As this change of substrate specificity was made at the cost of activity, the aim was now to further improve the overall activity of the enzyme.
On the other hand, the log odds ratio log(ψ 01) increases as the sensitivity (i.e., π a1) of alcohol increases when the specificity (i.e., π a0) is kept fixed; the log odds ratio log(ψ 01) appears less sensitive to the change of the specificity (i.e., π a0) when the sensitivity (i.e., π a1) is given.
This transfer would have had to happen after the change of substrate specificity.
