Exact(3)
The scaffolds were characterized at different degradation times by a series of analysis including pH changes of solutions, weight loss, swelling ratio, SEM, ATR-FTIR, DSC, TGA and mechanical properties.
Complete replacement of phosphate buffer saline two times a week prevented pH changes of solutions that could result from increase of degradation products.
Tl+-bound GltPh-R397A crystals were obtained by soaking GltPh-R397A apo crystals in several changes of solutions containing 18 20% PEG 400, 0.1 M citric acid/Tris pH 4.5, 0.1 M TlNO3, 7 mM n-decyl-α-D-maltopyranoside and 10% glycerol.
Similar(57)
The changes of solution color and the peak intensities were readily obtained in the Hg2+ detection.
These reactions result in the changes of solution conductivity that can be registered by the conductometric transducer.
Following a 1 h wash with phosphate-buffered saline (with several changes of solution), the oocytes were incubated with anti-mouse IgG conjugated to FITC or rhodamine red (1/200 dilution) for 2 h at room temperature.
After incubation, the preparations were extensively dialysed against PBS at 4°C with three changes of solution.
Samples were then washed for 10 hr in PBST with a minimum of 3 changes of solution.
Briefly, gel pieces were destained by incubating in 50% acetonitrile 15 min at room temperature with two to three changes of solution.
The slow rehydration-dehydration caused less destruction of testicular and epididymal tissue than the rapid single-step changes of solution and has an overall better morphological detail preservation compared to the rapid ETOH-PBS solution change.
Indeed, with the 1 mm discs rocking may be dispensed with if extra changes of solution are added to the protocol, as the disturbance from the extra solution changes is sufficient agitation.
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