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The changes of solution color and the peak intensities were readily obtained in the Hg2+ detection.
These reactions result in the changes of solution conductivity that can be registered by the conductometric transducer.
Following a 1 h wash with phosphate-buffered saline (with several changes of solution), the oocytes were incubated with anti-mouse IgG conjugated to FITC or rhodamine red (1/200 dilution) for 2 h at room temperature.
After incubation, the preparations were extensively dialysed against PBS at 4°C with three changes of solution.
Samples were then washed for 10 hr in PBST with a minimum of 3 changes of solution.
Briefly, gel pieces were destained by incubating in 50% acetonitrile 15 min at room temperature with two to three changes of solution.
Similar(49)
The scaffolds were characterized at different degradation times by a series of analysis including pH changes of solutions, weight loss, swelling ratio, SEM, ATR-FTIR, DSC, TGA and mechanical properties.
Complete replacement of phosphate buffer saline two times a week prevented pH changes of solutions that could result from increase of degradation products.
The AuNP enables to strongly interact with Pb2+ that leads to distinct color change of solution.
The change of solution color from yellow to black indicates the formation of bimetallic compound.
The formation of iron nanoparticles were indicated by the color change of solution from light pink to black.
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