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It provided a great guarantee to monitor slight changes of peptide in explosive detections.
Many natural enzymes control access of substrates to the active sites by the conformational changes of peptide loops; this phenomenon has been referred to as "gating".
However, the omitted proportion may also be valuable, in particular for the open search where the changes of peptide fragmentation patterns caused by some unanticipated PTM s) should be considered.
Second, we examined the top-scoring site pairs, and compared them with known SDRs from a mutagenesis study [ 13] in which ten sites of the ERBB2IP-1 domain were mutated and the corresponding changes of peptide specificity reported.
Thus HCMV infection must lead to extensive qualitative and quantitative changes of peptide ligands presented by MHC I and MHC II molecules which are not restricted to the emergence of viral epitopes but could include also novel endogenous self-epitopes on the cell surface.
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The conformational changes of peptides induced upon binding of Zn2+ to histidine were analyzed using circular dichroism spectroscopy, which revealed peptides, HA and HG, to be good candidates for further analyses.
The median fold-change of peptides associated to the same proteins was calculated.
Direct comparison of labeled versus unlabeled samples is possible, but as for peptide mass shifts (see " Mass change of peptides and fragments"), sample complexity and low abundance of peptides of interest can make retention time shifts hard to detect.
It is also noteworthy that the peptide dissociation patterns in MS/MS may be affected by oxidatively modified residues, which must be taken into account during the interpretation of MS/MS spectra [ 148] (see " Mass change of peptides and fragments").
Apparent, because upon peptide binding to the membrane, two processes occur: mass and structural changes of the peptide itself and mass and structural changes of the lipid bilayer to accommodate the peptide.
Micellar aggregation accompanying small conformational changes of the peptide from random coils to β-sheets is suggested as the sol-to-gel transition mechanism of the PEG-PAF aqueous solution.
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CEO of Professional Science Editing for Scientists @ prosciediting.com