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After staining with a DAPI solution (10 μg/ml) for 10 min in the dark at room temperature, the stained cells were washed twice with PBS to remove the excess DAPI and the changes of nucleus were examined with excitation of 330 to 380 nm and emission of 420 nm using a fluorescence microscope (Olympus, Tokyo, Japan).
The GO term analysis showed that great changes of nucleus and microtubules of ovary cells might take place mainly by abnormal transcription regulation, DNA repair, and ligand binding affected by age.
The present study was conducted to address whether the intervertebral disc of rabbit could be considered (i) as a valuable model to provide new insights into the tissue and cellular changes of Nucleus pulposus aging and (ii) as an appropriate tool to investigate the efficacy of Nucleus pulposus cell-based biotherapies.
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The changes of nucleus-independent chemical shifts (NICSs) as a method examining conjugational degree are reported.
For the assessment of apoptosis, morphological changes of nuclei were visualized following DNA staining by the fluorescent dye, DAPI.
This translocation is associated with triggering of morphological changes of nuclei, although the mechanisms are not established.
Accurate measurements of nuclear deformation, i.e., structural changes of the nucleus in response to environmental stimuli, are important for signal transduction studies.
In this study, we explore the structural changes of the nucleus of human mesenchymal stem cells as an effect of topographical cues.
It would be extremely difficult to argue that NPs remain extracellular and follow the changes of the nucleus while remaining fixed on the cell membrane.
Disc degeneration is generally initiated from morphological and compositional changes of the nucleus pulposus [ 8].
The podocyte revealed microvilli alteration, mesenchyme fibrosis and plentiful leaflet changes of cell nucleus.
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CEO of Professional Science Editing for Scientists @ prosciediting.com