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This translocation is associated with triggering of morphological changes of nuclei, although the mechanisms are not established.
For the assessment of apoptosis, morphological changes of nuclei were visualized following DNA staining by the fluorescent dye, DAPI.
Since the early embryonic events are closely related to the position, morphological variations, number changes of nuclei, and so on, automated detection of nuclei in DIC microscope images plays an essential role for understanding animal development.
Changes of nuclei in isolated two adjacent cells.
This protective effect on neuronal cell death was also detected in the activity of caspase-3 (Fig. 4D) and the apoptotic morphological changes of nuclei (Figure S3).
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The changes of nucleus-independent chemical shifts (NICSs) as a method examining conjugational degree are reported.
After staining with a DAPI solution (10 μg/ml) for 10 min in the dark at room temperature, the stained cells were washed twice with PBS to remove the excess DAPI and the changes of nucleus were examined with excitation of 330 to 380 nm and emission of 420 nm using a fluorescence microscope (Olympus, Tokyo, Japan).
The GO term analysis showed that great changes of nucleus and microtubules of ovary cells might take place mainly by abnormal transcription regulation, DNA repair, and ligand binding affected by age.
The present study was conducted to address whether the intervertebral disc of rabbit could be considered (i) as a valuable model to provide new insights into the tissue and cellular changes of Nucleus pulposus aging and (ii) as an appropriate tool to investigate the efficacy of Nucleus pulposus cell-based biotherapies.
We further analyzed morphological changes of the nuclei using NeuN/DAPI staining.
The model cannot yet describe the complex morphological changes of mitotic nuclei, and we discuss possible reasons for this.
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