Sentence examples for changes of media from inspiring English sources

Exponentially growing Hela cells were cultured for five days in medium containing 30 ng/ml TSA (Sigma) or 1 micromolar 5-azacytidine (Sigma), with daily changes of media.

Cells were then grown with daily changes of media for 7 days.

Cells were grown with daily changes of media and the amounts of cell-associated PrPSc were evaluated after 7 days.

TI biopsies were suspended in calcium and magnesium free medium with 2 mM EDTA and 1 mM DTT and agitated for 30 min with three changes of media.

Changes of media were performed every two to three days, with the recombinant protein being also freshly added to the respective controls.

E1 is active over a broad range of pH and still quite active at a low pH (~pH 5.5) [ 21, 22], which is similar to the optimum pH range for CelA and covers the spectrum of pH changes of media acidification during C. bescii growth (pH drop from pH 7.2 to ~5.0) [ 24].

In all studies, a piece (1/8) of an algae-coated fiberglass filter was placed at the test start and following every change of media into each beaker to provide food to the test animals.

Change of media was done every three days.

Adenoviruses encoding HA (KAN-1), NP (KAN-1) and M2 (KAN-1) were transfected into A549 cells for 48 hours followed by a change of media.

Cells were harvested 48 hours transfection (i.e. 24 hours after change of media) and RNAs isolated according to the method we described previously [48].

The heterogeneous mixture was allowed to grow for 96 hours to eliminate any artifacts brought on by sudden change of media for HeLa cells.