After addition of each solution to cell culture, changes of gene expression were studied.
However, comparison with previous functional studies indicated that not all changes of gene expression are relevant for the pathophysiology.
Epigenetic alterations include DNA methylation, histone modifications, and miRNA in the permanent changes of gene expression, which controls the leukemia phenotype.
The changes of gene expression related to apoptosis cascade, endoplasmic reticulum (ER) stress, oxidative stress, adhesion molecules, and calcium-handling proteins were depicted in Fig. 5.
Second, we explored the changes of gene expression in manipulated hierarchies.
Changes of gene expression were determined using the 2−ΔΔCt method [36].
The coordinated changes of gene expression may result from several causes.
Among possible point mutations in ORFs, nonsense mutations cause the most drastic changes of gene products.
Thus, it is easy to see how relevant changes of gene expression in a minority population could be missed.
This Ca2+ influx initiates a variety of intracellular signalling pathways, possibly also involving changes of gene expression [60], [61].
Thus, up and down changes of gene expression by a balanced diet (normal mouse chow) may not be reproduced by a simple diet of 100% glucose.
