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This approach is an extension of metabolic control analysis (MCA), valid for large changes of enzyme and metabolite levels.
Hence, it was concluded that the changes of enzyme expression involved in pyruvate catabolism were negligible in response to growth (Rydzak et al. 2009).
Compared with WT, the mutations indeed resulted in the alteration of both binding energy and conformation, which collectively led to the changes of enzyme enantioselectivity.
In others, large changes of enzyme function, in terms of both overall chemistry and substrate specificity, have been brought about by significant changes in catalytic machinery.
The industrial cellulases available on the industrial enzyme market from different makers and sources vary significantly in hydrolysis and ethanol, and finally lead to the changes of enzyme cost.
When enzymes immobilized on support, materials create a kind of protection and provide a resistance against the conformational changes of enzyme structure, which results in an increased thermal stability of laccase nano-conjugates [31].
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Altered drug ADME during inflammation has long been primarily ascribed to changes of enzymes in drug metabolism.
Conformational changes of enzymes and their substrates during the catalytic cycle have now been identified for many systems.
In this study, expression changes of enzymes involved in host lipid metabolism provide indirect evidence suggesting that symbiotic anemones are indeed processing more lipids than are aposymbiotic anemones.
In this paper, we use the phrasing "up- or down-regulation" to describe engineering modifications that result in expression level changes of enzymes or groups of enzymes regardless of the method.
In general, however, the changes of enzymes in the TCA cycle and glycolysis cannot fully explain the difference of organic acid and sugar contents in Temple compared to Murcott.
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