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Shah et al. (2006) monitored the hydrolysis of p- nitrophenyl palmitate (PNP) catalyzed by CLEAs of lipase from Pseudomonas cepacia through changes of absorbance at 410 nm.
In the presence of an antioxidant, the purple color intensity of DPPH solution decays and the changes of absorbance are followed spectrophotometrically at 517 nm.
The spectroelectrochemical results prove clearly the existence of two electroactive moieties integrated in the polymeric lattice, the phenothiazine ring (detected by changes of absorbance at 590 and 685 nm) and the newly formed covalent links which fixes the monomers in the backbone of the polymer (detected by changes of absorbance at 460 and 875 nm).
The method is based on the reaction between Brilliant Green (BG) as a colored reagent and sulfite and/or sulfide in buffered solution (pH 7.0) and monitoring the changes of absorbance at maximum wavelength of 628 nm.
The kinetic methodology based on the difference of reaction rates, is based on the reaction between a common oxidizing agents such as tris(1,10-phenanthroline) and iron III) complex (ferriin, [Fe (phen 3]3+) in the presence of citrate and spectrophotometrically, monitoring the changes of absorbance at the maximum wavelength of 511 nm.
The values of the ratios between the propagation rate constant and the square root of the termination rate constant (kp/kt1/2) were determined by UV spectroscopy by measuring the changes of absorbance with time at several wavelengths in the range 275 285 nm, where appropriate changes in absorbance were observed.
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Figure 3 Change of absorbance with wavelength under different polymerization conditions.
The change of absorbance was used as a criterion to evaluate the reduction efficiency.
The change of absorbance at 340 nm was monitored for 40 s.
Change of absorbance per minute was measured and reported as µM/mg protein/min (Aebi 1984).
The progression of the reaction can be measured by monitoring the change of absorbance of a pH indicator (phenol red).
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