Sentence examples for changes in the kinome profile from inspiring English sources
As the infection progressed more changes in the kinome profile were detected, creating a greater distance in the cluster.
Additional file 8 (.pdf) shows unsupervised clustering of technical replicates used in the kinome profiling experiment.
Interestingly, 6 hours after infection, the kinome profile resembled that of the control more than the profile at 3 hours.
The kinome profile dendogram (Figure 2a) generated from lung samples harvested 6 hours after infection was more closely related to control than the kinome profile obtained after 3 hours.
Hierarchical clustering of the kinome data derived from human and porcine PBMCs showed that the kinome profiles clustered in a species-specific manner, suggesting that kinotype analysis could provide critical information regarding cell processes or signalling pathways that are differentially modulated across similar animal species.
Inclusion parameters for each of the kinome profiles are described in supplemental data, Table S4.
In order to identify peptides driving the differences between the kinome profiles of different individuals, the 36 normalized intensity values for a given individual (4 samples per individual × 9 technical replicates per sample) were averaged for each peptide.
In order to detect overactive kinases in osteosarcoma, which may be potential targets for treatment, we identified the most significant pathways by a single-way analysis of the kinome profiling data.
Unsupervised pathway analysis on the kinome profiling results returned the IPA pathway PI3K/Akt signaling as the most significantly affected pathway in osteosarcoma cells and the AMPK pathway as second most significantly affected pathway (Additional file 9).
As with the hierarchical clustering, there was a strong trend for the kinome profiles to segregate on the basis of individual.
In the second kinome profiling experiment we compared lysates of untreated cells with lysates of cells treated with MK-2206.
