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Track changes in the clownfish eggs.
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A recent study by Kim et al. (Kim et al., 2012) showed that GnRH peptides play important roles in the regulation of the hypothalamic-pituitary-gonadal axis and probably control gonad development and sex change in the cinnamon clownfish (Amphiprion melanopus).
Recognize the changes in clownfish behavior that signal spawning.
We find several deep cytonuclear discordances in the clownfish.
Our results show instances of cyto-nuclear incongruence in the clownfish phylogenetic tree, which suggests several hybridisation events.
In other words, the high slope value of the correlation between fish size and dominant frequency in the clownfish indicates that the size of the emitter can be assessed by the receiver and so be used in sonic communication.
This node has been previously linked with a burst of diversification rate in the clownfish clade [ 22, 23] and may also be mainly responsible for the increase in rate of morphological evolution found in the group [ 18].
It should be noted that the extinction rate also increases in the clownfishes.
To verify that the elevated rates found in the clownfishes were not due to the relatively short branches of the clownfish clade we simulated a continuous trait under a BM model with a single rate on our phylogenies.
In this case, only the standard length, body ratio, soft anal fin-rays and gill rakers had a faster evolutionary rate in the clownfishes.
In this context, we study hybridisation patterns in the clownfishes (or anemonefishes; Pomacentridae), a monophyletic clade which maintains mutualistic interactions with sea anemones [ 17].
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