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The aim of this work was to assess the changes in the chemical profile of methanolic extract of tomato roots by high-resolution electrospray source with ion trap time-of-flight (IT-TOF) detector (ESI-IT-TOF MS).
Thus, changes in the chemical profile of these two major classes of substances may induce differences in the attractiveness of nectar.
As an example, the injection of ringer or LPS in honeybee workers induced significant changes in the chemical profile of cuticular hydrocarbons that affect social interactions between unhealthy and healthy nestmates (Richard et al. 2008; Richard et al. 2012).
Indeed, the coating of a social insect with exogenous chemicals is known to induce aggression in nestmates since this induces global changes in the chemical profile of the coated individual, although coating ants with the cuticular hydrocarbons of their nestmates does not elicit an aggressive response [ 55].
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For a more thorough description and discussion of the changes in the chemical profiles of the treated soil, the reader is referred to the original publication.
Finally, we treated healthy worker bees with the cuticular extracts of control or LPS-injected workers and examined their interactions with untreated nestmates, to determine if the changes in the chemical profiles could indeed be responsible for the altered social interactions.
In addition to the convergence of odours that occurred between colonies following treatment with one another's soil, we also found that non-nestmate soil induced a non-colony-specific change in the chemical profiles of treated ants.
We also found a change of the chemical profile with age which is consistent with previous studies (nurse vs forager, see [ 43]).
Variation in the chemical profile of essential oils could influence their biological activities.
It is possible in our experiments that coating individuals with extracts caused additional changes to the chemical profiles that elicited a stronger behavioral response from the healthy nestmates.
This phenologic changes of the chemical profiles coincided with the morphological changes of the regular form from mycelium to fruiting body (Chu et al. 2010).
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