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Recent studies have revealed that mating can alter starvation resistance in female D. melanogaster, but little is known about the behavioral and physiological mechanisms underlying such mating-mediated changes in starvation resistance.
Although drugs such as hydroxyurea can be used to effectively synchronize cells at the level of DNA synthesis [20], [43], [44], the mechanism of action (deprivation of nucleotide precursors via DHFR-TS inhibition) is somewhat similar to the general nutrient deprivation technique we had already employed, and might similarly cause changes in starvation- or stress- regulated transcripts.
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Notably, temperature did not change in this starvation model.
It is known that many genes respond to various environmental changes, including changes in temperature and starvation.
We previously used microarray analyses to explore genome-wide transcript changes in response to starvation in larvae (Li et al., 2010).
An effective way to identify the relationships between major metabolic pathways and body processes is to examine changes in metabolism during starvation.
Examples from the literature are used to underscore areas in which reporting and statistical practices, particularly those involved with starvation-induced changes in body composition and starvation-induced hypometabolism can be improved.
In order to shed light on the DNA methylation pathway mediating Pi starvation-induced changes in DNA methylation, the phosphate starvation experiment was repeated using an RNAi line that knocks down DCL3a, a key factor involved in the canonical RdDM pathway.
This study provides the first insights into the intestinal microbiome and its changes under starvation in fish.
Hence, it is not surprising that also starvation induced changes in the intracellular Ca2+ homeostasis and that starvation-induced autophagy was also abolished by BAPTA-AM.
We therefore also examined the changes in endogenous LC3 after starvation in these cells.
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