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Similar changes in staining patterns were seen in HSV1 infected human retinal epithelial cells (ARPE-19), Hela cells, and neuronal cell lines (PC12 and SHSY 5Y), although some cells retain the peri-nuclear position of the Golgi longer after infection than others.
Immunohistochemical staining for PTEN revealed no obvious changes in staining intensities in normal, FEA, DCIS and IDC.
Any procedural changes in staining the slides will give inconsistent values; therefore, all slides in a study should be ideally stained at the same time.
Subtle changes in staining bordering the ablation area are evident, double arrows) and are typical of thermal damage from laser energy.
While this is in principle a valid point, there are in fact no discernable changes in staining intensity but rather in staining localization.
Therefore, we performed immunostaining for ionized calcium-binding adapter protein 1 (Iba1), which exclusively labels ramified microglia and is useful in determining activation of microglia via changes in staining intensity, soma size and shape, and ramification of processes [ 25, 26].
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Co-staining with CD63, a tetraspanin that is abundant in late endosomes and lysosomes, revealed an interesting change in staining pattern in cells expressing mutant CHMP2B.
Rapamycin was also used to enhance autophagy and resulted in a similar change in staining pattern as noted for serum starvation (Figure 10B).
At this early time-point of recovery, we did not observe any change in staining for the oligodendrocyte marker Rip within the subcortical white matter or SVZ (Data not shown [26]).
This change in staining pattern indicates a profound pathology at this stage.
We quantified this change in staining intensity by measuring the mean gray value of the images.
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